A projection of a mouse C2C12 nucleus at prophase created with 3D-SIM. Condensed chromosomes (stained with DAPI; shown in red) are visible in high detail, surrounded by the nuclear lamina (Lamin B ...

A cultured human cell (HeLa cell) in prophase. Chromosomes are shown in shades of red, microtubules are marked in blue. 3D image taken with a confocal microscope. Credit: Monica Gobran, Max Planck ...

A final alternative is to place the entire microscope in an environmental room of constant temperature. Prophase cells may revert to interphase if they are damaged by light. This may occur because ...