In general, the ratio of fixative to tissue (vol:vol) should be 15:1 to 20:1. Dense (skin, bone) and bloody (liver, spleen) tissues are hardest to fix. If the amount of fixative is inadequate, or the ...

Tissue staining and histology. Coomassie blue (30 μl; BioRad, Hercules, California) was spread across MEA pre-treated skin or was directly applied to shaved skin. Unbound dye was blotted dry and ...