A short hairpin RNA or small hairpin RNA (shRNA/Hairpin Vector) is an artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi).

siRNA/shRNA/oligo Optimal Design considering off-target: Besides intended gene down-regulation, shRNAs can introduce off-target gene silencing. Our program design shRNA with higher on-target potency, and less off-target effect.

We provide suggestions for designing shRNA targets and controls, a protocol for sequencing through the secondary structure of the shRNA hairpin structure, and protocols for packaging and delivery of shRNA lentiviral particles.

Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more.

Short interspersed nuclear elements (SINEs) are non-autonomous, non-coding transposable elements (TEs) that are about 100 to 700 base pairs in length. [1] . They are a class of retrotransposons, DNA elements that amplify themselves throughout eukaryotic genomes, often through RNA intermediates. SINEs compose about 13% of the mammalian genome. [2]

Research has shown that siRNA and shRNA are instrumental in the RNA interference (RNAi) pathway, leading to the silencing of specific genes. siRNA is typically introduced directly into cells, whereas shRNA is expressed from a DNA vector, making it …

Oct 29, 2022 · Two key approaches to RNAi that have gained substantial interest for use in gene silencing are the double-stranded small interfering RNAs (siRNAs) and the vector-based short hairpin RNAs (shRNAs). While both siRNAs and shRNAs (Figure 1) can be used for protein knockdown, there are differences in their mechanisms of action (Figure 2).

Dive into the world of RNA interference with our comprehensive guide on siRNA, shRNA, and miRNA. Learn how these molecules induce gene silencing, their structural differences, and their applications in modern biology.

Jul 25, 2009 · Methods of mediating the RNAi effect involve small interfering RNA (siRNA), short hairpin RNA (shRNA) and bi-functional shRNA. The simplicity of siRNA manufacturing and transient nature of the effect per dose are optimally suited …

Santa Cruz Biotechnology now offers siRNA and shRNA (plasmid and lentiviral) gene silencers targeted to all (100%) known human and mouse genes. siRNAs are provided as pools of 3 target specific 19-25 nt siRNAs designed to knock down expression of specific gene of interest.